Optoelectron. Semicond. Tech. 56, 134-155 (2021)

Yu. M. Shirshov, K. V. Kostyukevych, R. V. Khistosenko, N. Ya. Gridina, S. A. Kostyukevych, A. V. Samoylov, Yu. V. Ushenin

Optical control of the interface between gold surface and blood cell samples

The optical properties of blood (spectra of the extinction coefficient, k, refractive index, n, etc.) carry important diagnostic information and are usually monitored using bulk samples. In this work, attention is drawn to the interface between the blood volume and the surface of glass or thin gold films on it, where the refractive index may differ from the bulk one. We draw attention to the relationship between two effects – SPR and TIR. It is shown that if the named effects are measured for two different external media 0 and 1 with different refractive indices, then the values of the angles SPR and TIR will be linearly related by the empirical formula SPR1=SPR0+TIR1- TIR0)*K, where the coefficient K depends on the thickness of the transition layer di between the surface and the volume of the liquid medium (suspension). Numerical calculation of K (di) for gold films shows that K = 1.6 at di = 0 and monotonically decreases to 0.01 with an increase in di to 300 nm (and further to 0). Measurement of the angular dependences of reflection, R(), on (1) 100% hematocrit blood samples, (2) hemolyzed samples and (3) washed erythrocytes with dilutions with a buffer solution. It was shown that all samples exhibit a minimum SPR, but the TIR angle can be measured only for blood samples with destroyed membranes (hemolyzed), buffer solution and plasma. The n-value for hemolyzed blood is 1.3505, which is indicative of a low hemoglobin content in the sample. At the same time, di for a sample of 100% hematocrit was 60-105 nm, which indicates a strong deformation of erythrocytes in the form of polyhedrocytes and their dense packing after centrifugation. Washing the cells with a buffer increases di to 280 nm and more and practically eliminates blood cells from the SPR sensitivity region. The reason for this may be that in the blood of 100% hematocrit, erythrocytes are in the form of polyhedrocytes tightly adhering to the gold surface, while as a result of washing and diluting with a buffer solution, the cells relax back into discocytes. As a result, the containing hemoglobin erythrocyte cytoplasm moves away from the surface at a distance di> 300 nm into the suspension volume and leaves the area of the enhanced plasmon-polariton field.

Keywords: surface plasmon resonance (SPR), total internal reflection, (TIR), refractive index, erythrocytes, hematocrit.


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Ю. М. Ширшов, К. В. Костюкевич, Р. В. Христосенко, Н. Я. Грідіна, С. А. Костюкевич, Ю. В. Ушенін, А. В. Самойлов

Оптичний контроль межі розподілу між поверхнею золота та зразками клітин крові

Звернено увагу на оптичні властивості межі розподілу між плоскою поверхнею скла чи плівки золота та суспезією клітин крови, що вміщує інформацію щодо форми власне клітин крови – еритроцитів. З метою визначенния товщини перехідного шару між поверхнею та об’емом суспензії було притягнуто порівняння кутів поверхного плазмонного резонансу SPR та повного внутріш-нього відбивання TIR . Ми встановили розрахунковим шляхом, що для двох зовнішніх середовищ з відмінніми показниками заломлення між кутми SPR та TIR існує лінійне співвідношення. Коефіціент нахилу цього співвідношення дозволяє визначити товщину перехідного шару, якщо він є тоншим за 300 нм. Експеримент проведено в геометрії Кретчмана для зразків повної крови 100% гематокриту в нативномуі, а також гемолізованиму станах. Також використано зразки еритроцитів які промито та розбавлено в буферному розчині. Показано, що еритроцити в нативних зразках 100% гематокриту щільно прилягають до золотої поверхні (на відстані 80-100 нм), що свідчить про сильну деформацію клитин які після цетрифугування перебувають в формі поліедро-цитів з низкою плоских ділянок на поверхні. Промивання клітин в буферному розчині та розве-дення призводить до релаксації форми еритроцитів у звичайну дискоцитну форму та віддаляє цитоплазму еритроцита від поверхні завдяки збвльшення радіусу викривлення мембрани.

Ключові слова: поверхневий плазмонний резонанс (ППР), повне внутрішне відбиття (ПВВ), показник заломлення, еритроцити, гематокрит.